Title : Forming of branching blood vessels using roll porous scaffold 3D bioprinting technology
Abstract:
The leading cause of premature end of life worldwide is cardiovascular disease. Deep vein thrombosis requires emergency medical attention. Currently, the preferred treatment for thrombosed vessels is usually to replace the blood vessels with the patient's own vessels. For example, to "restore" the most commonly damaged aorta, surgeons use the "less important" saphenous vein of the leg. However, with age, all human blood vessels wear out, and using this vein becomes impractical or impossible in approximately 20% of cases. Accordingly, the problem of manufacturing blood vessels is acutely relevant. A new technology to producing branched blood vessels is proposed. The Roll Porous Scaffold 3D bioprinting technology is designed to form complex biological objects using multiple fixed linear inkjet heads. In particular, heads like these “KJ4B-1200”, “KJ4C-0360” and “KJ4A-0300, KJ4B-0300” with resolution of 1200, 360 and 300 DPI accordingly from the company Kyocera with hundreds of thousands of nozzles operating at a frequency up to 100 kHz. The increase in print density by orders of magnitude is achieved through apply of a filter layer and an increase in exposure heads. The estimated performance of the new method is >1.7 L per hour at a layer thickness of 18 μm. The acceleration occurs because no time is required for the scaffold to form. The formation of the organoid occurs in a pre-prepared biodegradable mesh scaffold using a perforated laser. Films with cells of ~60−100 μm, boundaries of ~20−45 μm respectively and ensuring almost 100% cell viability were produced. Newly developed materials and methods can be applied to significantly accelerate the operation of traditional extrusion, laser and inkjet bioprinter.
